Isoberlinia tomentosa, a medicinal plant commonly found in the southern part of Borno State, Nigeria, has been traditionally used to treat various ailments, including diarrhea, malaria, rheumatic fever, strep throat, urinary tract infections, vaginal yeast infections, and dysentery. This research aimed to assess the in vitro antimicrobial activity and phytochemical composition of the ethyl acetate and n-butanol portions of Isoberlinia tomentosa stem bark extracts. The plant-based chemical analysis showed that the n-butanol portion contained flavonoids, cardiac glycosides, terpenoids, tannins, saponins, and carbohydrates. In contrast, the ethyl acetate portion contained tannins, carbohydrates, terpenoids, flavonoids, cardenolides, and cardiac glycosides, but no saponins were detected. Notably, anthraquinone glycosides and alkaloids were also not detected. The antimicrobial screening demonstrated that Isoberlinia tomentosa exhibited significant inhibitory activity against certain test organisms. Specifically, the ethyl acetate portion (500 mg/mL) showed pronounced inhibition against Streptococcus pyogenes, Salmonella typhi. and Staphylococcus aureus, Furthermore, the n-butanol portion (500 mg/mL) exhibited broad-spectrum inhibition against all tested bacteria and fungi, including Salmonella typhi Staphylococcus aureus, Bacillus subtilis Streptococcus pyogenes, Escherichia coli,, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Candida albicans. These results imply that the stem bark extract of Isoberlinia tomentosa has antibacterial and antifungal properties, affirming its historical application in treating various illnesses.
| Published in | Science Journal of Chemistry (Volume 13, Issue 6) |
| DOI | 10.11648/j.sjc.20251306.13 |
| Page(s) | 185-193 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2025. Published by Science Publishing Group |
Isoberlinia tomentosa, Phytochemical Screening, Antimicrobial, in vitro, Microorganisms
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APA Style
Dawa, S. I., Yakubu, J., Adam, S., Mamza, U. T., Honda, J. T. (2025). Phytochemical Screening and Comparative Study of the Antimicrobial Activity of n-Butanol and Ethyl Acetate Fractions of Isoberlinia tomentosa Stem Bark Extract. Science Journal of Chemistry, 13(6), 185-193. https://doi.org/10.11648/j.sjc.20251306.13
ACS Style
Dawa, S. I.; Yakubu, J.; Adam, S.; Mamza, U. T.; Honda, J. T. Phytochemical Screening and Comparative Study of the Antimicrobial Activity of n-Butanol and Ethyl Acetate Fractions of Isoberlinia tomentosa Stem Bark Extract. Sci. J. Chem. 2025, 13(6), 185-193. doi: 10.11648/j.sjc.20251306.13
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Download@article{10.11648/j.sjc.20251306.13,
author = {Samuel Ibrahim Dawa and James Yakubu and Sheriff Adam and Umar Tanko Mamza and Joel Tari Honda},
title = {Phytochemical Screening and Comparative Study of the Antimicrobial Activity of n-Butanol and Ethyl Acetate Fractions of Isoberlinia tomentosa Stem Bark Extract},
journal = {Science Journal of Chemistry},
volume = {13},
number = {6},
pages = {185-193},
doi = {10.11648/j.sjc.20251306.13},
url = {https://doi.org/10.11648/j.sjc.20251306.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjc.20251306.13},
abstract = {Isoberlinia tomentosa, a medicinal plant commonly found in the southern part of Borno State, Nigeria, has been traditionally used to treat various ailments, including diarrhea, malaria, rheumatic fever, strep throat, urinary tract infections, vaginal yeast infections, and dysentery. This research aimed to assess the in vitro antimicrobial activity and phytochemical composition of the ethyl acetate and n-butanol portions of Isoberlinia tomentosa stem bark extracts. The plant-based chemical analysis showed that the n-butanol portion contained flavonoids, cardiac glycosides, terpenoids, tannins, saponins, and carbohydrates. In contrast, the ethyl acetate portion contained tannins, carbohydrates, terpenoids, flavonoids, cardenolides, and cardiac glycosides, but no saponins were detected. Notably, anthraquinone glycosides and alkaloids were also not detected. The antimicrobial screening demonstrated that Isoberlinia tomentosa exhibited significant inhibitory activity against certain test organisms. Specifically, the ethyl acetate portion (500 mg/mL) showed pronounced inhibition against Streptococcus pyogenes, Salmonella typhi. and Staphylococcus aureus, Furthermore, the n-butanol portion (500 mg/mL) exhibited broad-spectrum inhibition against all tested bacteria and fungi, including Salmonella typhi Staphylococcus aureus, Bacillus subtilis Streptococcus pyogenes, Escherichia coli,, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Candida albicans. These results imply that the stem bark extract of Isoberlinia tomentosa has antibacterial and antifungal properties, affirming its historical application in treating various illnesses.},
year = {2025}
}
TY - JOUR T1 - Phytochemical Screening and Comparative Study of the Antimicrobial Activity of n-Butanol and Ethyl Acetate Fractions of Isoberlinia tomentosa Stem Bark Extract AU - Samuel Ibrahim Dawa AU - James Yakubu AU - Sheriff Adam AU - Umar Tanko Mamza AU - Joel Tari Honda Y1 - 2025/12/08 PY - 2025 N1 - https://doi.org/10.11648/j.sjc.20251306.13 DO - 10.11648/j.sjc.20251306.13 T2 - Science Journal of Chemistry JF - Science Journal of Chemistry JO - Science Journal of Chemistry SP - 185 EP - 193 PB - Science Publishing Group SN - 2330-099X UR - https://doi.org/10.11648/j.sjc.20251306.13 AB - Isoberlinia tomentosa, a medicinal plant commonly found in the southern part of Borno State, Nigeria, has been traditionally used to treat various ailments, including diarrhea, malaria, rheumatic fever, strep throat, urinary tract infections, vaginal yeast infections, and dysentery. This research aimed to assess the in vitro antimicrobial activity and phytochemical composition of the ethyl acetate and n-butanol portions of Isoberlinia tomentosa stem bark extracts. The plant-based chemical analysis showed that the n-butanol portion contained flavonoids, cardiac glycosides, terpenoids, tannins, saponins, and carbohydrates. In contrast, the ethyl acetate portion contained tannins, carbohydrates, terpenoids, flavonoids, cardenolides, and cardiac glycosides, but no saponins were detected. Notably, anthraquinone glycosides and alkaloids were also not detected. The antimicrobial screening demonstrated that Isoberlinia tomentosa exhibited significant inhibitory activity against certain test organisms. Specifically, the ethyl acetate portion (500 mg/mL) showed pronounced inhibition against Streptococcus pyogenes, Salmonella typhi. and Staphylococcus aureus, Furthermore, the n-butanol portion (500 mg/mL) exhibited broad-spectrum inhibition against all tested bacteria and fungi, including Salmonella typhi Staphylococcus aureus, Bacillus subtilis Streptococcus pyogenes, Escherichia coli,, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Candida albicans. These results imply that the stem bark extract of Isoberlinia tomentosa has antibacterial and antifungal properties, affirming its historical application in treating various illnesses. VL - 13 IS - 6 ER -
Department of Chemistry, Kashim Ibrahim University, Maiduguri, Nigeria
Department of Chemistry, University of Maiduguri, Maiduguri, Nigeria
Department of Chemistry, Kashim Ibrahim University, Maiduguri, Nigeria
Department of Chemistry, University of Maiduguri, Maiduguri, Nigeria
National Centre for Technology Management North East Zone, Modibbo Adama University, Yola, Nigeria
